International Journal of Advanced Sport Sciences Research

ASSR is an open access journal, aims at rapid publication of concise research papers of a broad interest in Physical education fields. Subject areas include all the current fields of interest represented by the Committees of the Design Scientific Renaissance. ASSR welcomes papers and articles in sport and physical education, fields of ASSR includes but not limited to: sport for all; Exercise physiology; Moths of training and coaching;Sport’s performance and analysis

Read More >


Journal of Advanced Computer Science and Technology Research

JACSTR is an open access journal, aims at rapid publication of concise research papers of a broad interest in computer science and information technology fields.

Read More >


Journal of Advanced Laser and Optics Research

JALOR aims at rapid publication of concise research papers of a broad interest in laser and optics fields. Subject areas include all the current fields of interest represented by the Committees of the Design Scientific Renaissance. JALOR accepts papers and articles in fields, including but not limited to the following: Actuator; Detectors; Ferroelectric And Ferromagnetic Materials; Filters; Holography; Laser Accessories And Optics

Read More >


Journal of Advanced Medical Research

Journal of Advanced Medical Research (JAMR) is an open access journal, provides rapid publication of various articles in the fields of Medical, Dentistry, Pharmacy, Comparative Veterinary and Medical sciences, and related disciplines. JAMR seeks to publish experimental and theoretical research results of outstanding significance in the form of original articles, reviews, case reports, short reports, or letters to the editor.

Read More >


Journal of Advanced Science and Engineering Research

JASER is an open access journal, aims at rapid publication of concise research papers of a broad interest in science and engineering fields. Subject areas include all the current fields of interest represented by the Committees of the Design Scientific Renaissance.

Read More >


Journal of Advanced Social Research

JASR is an open access journal, aims at rapid publication of concise research papers of a broad interest in social fields. JASR welcomes papers and articles in social fields, including but not limited to the following: Accounting; Applied Economics; Business Law; Business Management; Economics; Education Read More >

Journal of Purity, Utility Reaction and Environment

JMMR aims at rapid publication of concise research papers of a broad interest in marketing fields. JMMR welcomes papers and articles in marketing fields, including but not limited to the following: Consumer behavior; CRM; Customer Knowledge Management; Advertising economies; Consumer modeling; Marketing research; Interactive marke

Read More >


Journal of Purity, Utility Reaction and Environment

The Journal of Purity, Utility Reaction & ENVIRONMENT focuses upon six aspects of chemical engineering: chemical reaction engineering, environment chemical engineering, and materials synthesis and processing, catalyst surface reaction, optimization and control.

Read More >

Reader Comments

Tudied [31]; these were chosen for the current study. Behavioral testing commenced

by Fannie Barraza (2020-05-25)


Tudied [31]; these were chosen for the current study. Behavioral testing commenced at PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26475603 P10-11 and continued 3 times weekly until P43-44 for all kits (n = 16). Behavior tests were conducted prior to the daily drug administration to minimize the effect of lethargy in the MgSO4 recipients. The kits were not exposed to the test situations PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27788604 prior to testing. Open field behavior was assessed using two apparatuses. The first chamber was an enclosed square (44 ? 43 ? 29 cm) with 15 light beam sensors on each axis to quantitate ambulatory, vertical, and total movements (Opto-Varimex 3; Columbus InstrumentsAll paraffin blocks were sectioned coronally (6 m thickness) and stained with hematoxylin and eosin (H and E). The cerebrum at the level of the anterior horn of the lateral ventricles was stained with solochrome cyanine and counterstained with eosin for LGD-2226 visualization of myelin. Sections were immunostained with rabbit polyclonal anti-glial fibrillary acidic protein (GFAP; 1:10,000 dilution; DAKO Z0334; Glostrup, Denmark) to label astrocytes and reactive astrocytes. The primary antibody underwent 1.5 h incubation at room temperature. This was followed by incubation with appropriate biotinylated secondary antibody, followed by reaction with streptavidin-peroxidase, detection with diaminobenzidine (DAB, Sigma D5905), and finally counterstaining with hematoxylin. Negative controls were treated without the primary antibody. Corpus callosum thickness was measured using 100?ocular magnification at the sagittal midline and above the lateral angle of the anterior horn of the lateral ventricle. The second site was chosen because fragmentation of the midline region in some hydrocephalic brains preventing proper measurement of corpus callosumDi Curzio et al. Fluids Barriers CNS (2016) 13:Page 4 ofthickness. For comparative purposes, non-hydrocephalic ferrets from our previous experiment [27] were also examined.Myelin enzymes and enzymelinked immunosorbent assaysFrozen dorsal frontal and parietal cerebrum samples were homogenized using a radio immunoprecipitation assay (RIPA) buffer including protease inhibitors phenylmethylsulfonyl fluoride (PMSF) and aprotinin, which likely explain the lower myelin enzyme activities than were obtained previously [27]. Total protein quantification was determined with the Micro BCA (Pierce) Protein Assay kit (Thermo Scientific, Rockford, Illinois, USA). Dorsal frontal cerebrum homogenates were used to quantify myelin basic protein (MBP) and GFAP content by using ELISAs, as previously described in detail [27]. Colorimetric assays were performed in triplicate, and results are shown in g of MBP or GFAP per gram of protein and averaged per sample. Dorsal parietal cerebrum samples were used to quantify the enzyme activity of UDP-galactose:ceramide galactosyltransferase (CGalT) and glycerylphosphorylcholine phosphocholine phosphodiesterase (GPC-PP) using the artificial substrate p-nitrophenylphosphorylcholine (pNPP). Both are enzymes that are enriched in myelin. They were quantified in triplicate as described previously [32, 33].Atomic absorption spectroscopywere assessed using ANOVA and two-tailed t-tests for behavioral testing, ventricle size, histological data, and biochemical assays to compare MgSO4 and NaCl hydrocephalic treatment groups. Qualitative assessments for motor and behavioral development were analyzed separately from quantitative measures. Statistical analyses were conducted using the SPSS 19.0 software program.